TRPV1: Contribution to Retinal Ganglion Cell Apoptosis and Increased Intracellular Ca with Exposure to Hydrostatic Pressure
نویسندگان
چکیده
METHODS. trpv1 mRNA expression in RGCs was probed with the use of PCR and TRPV1 protein localization through immunocytochemistry. Subunit-specific antagonism (iodo-resiniferatoxin) and agonism (capsaicin) were used to probe how TRPV1 activation affects the survival of isolated RGCs at ambient and elevated hydrostatic pressure ( 70 mm Hg). Finally, for RGCs under pressure, the authors tested whether EGTA chelation of Ca improves survival and whether, with the Ca dye Fluo-4 AM, TRPV1 contributes to increased intracellular Ca .
منابع مشابه
TRPV1: contribution to retinal ganglion cell apoptosis and increased intracellular Ca2+ with exposure to hydrostatic pressure.
PURPOSE Elevated hydrostatic pressure induces retinal ganglion cell (RGC) apoptosis in culture. The authors investigated whether the transient receptor potential vanilloid 1 (TRPV1) channel, which contributes to pressure sensing and Ca(2+)-dependent cell death in other systems, also contributes to pressure-induced RGC death and whether this contribution involves Ca(2+). METHODS trpv1 mRNA exp...
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